Benserazide hydrochloride: white or off-white crystalline powder, easily soluble in water, slightly soluble in ethanol, insoluble in acetone. It is a decarboxylase inhibition drug.
Method name: Determination of Benserazide hydrochloride by high performance liquid chromatography
Application scope: This method is used to determine the content of Benserazide hydrochloride by high performance liquid chromatography.
This method is applicable to Benserazide hydrochloride.
Principle of the method: The mobile phase solution of the sample and the internal standard are made into the high performance liquid chromatograph for chromatographic separation, and the absorption values of benzisazide hydrochloride (C10H15N3O5-HCl) and the internal standard dopamine hydrochloride are detected at 220 nm with a UV absorption detector to calculate their contents.
2. trifluoroacetic acid
1.1 High performance liquid chromatograph
1.2 Chromatographic column
Octadecylsilane bonded silica gel as filler, the theoretical plate number should be not less than 1000 according to the calculation of benzyl hydrazine hydrochloride peak, and the separation of benzyl hydrazine hydrochloride peak and the peak of the internal standard substance should meet the requirements.
1.3 UV absorption detector
2. chromatographic conditions
2.1 Mobile phase: water trifluoroacetic acid + methanol = 1000 1 20
2.2 Detection wavelength: 220nm
2.3 Column temperature: room temperature